Showing posts with label Guess that Gizmo. Show all posts
Showing posts with label Guess that Gizmo. Show all posts

Saturday, October 4, 2008

Spread Plating and Guess That Gizmo

Before I showed you streak plating, that is a method that is often used when checking for contamination and is supposed to result in isolated colonies that originate from a single organism that lands on a spot and grows there until it forms a visible area of growth in the form of a dot. Spread plating is a very common method that is used to enumerate the bacteria in a sample. Most of my studies have to do with how well my bacteria survive in different environments and so I do dilutions to get a smaller number of bacteria per mililiter and spread plate the dilutions and see how many colonies I get, the concept behind said colonies is the same as for streak plating.

The two dilutions I commonly make are 1 in 100 (1 part of sample into 99 parts diluent) and 1 in 10. When I plate the sample I plate 0.1 ml so that adds another 1 in 10 dilution in the end. I multiply the number of colonies on the resultant plates by the number of times I diluted and it gives me the number of cells per ml in the original sample. Not diluted enough the bacteria form a smear across the plate, if too diluted then only a few colonies if any are present, I want approximately 20-250 colonies to have what is considered statistically significant results. And at risk of losing all of my beloved readers and having Baby Sibling make fun of how incredibly dorky I am I have made a video of me doing a dilution set and spread plating it:

Here are two plates, the one on the left I used the spread plating technique and the one on the right I streak plated:

You are still here??? Great! Now that you have gotten to laugh at me how about giving me a laugh or two (with you not at you), it is time for the next Guess That Gizmo!
Sorry about the not so sticky biohazard sticker, they just don't make 'em like they used to I guess:
And remember, this is a semester long contest so new readers can participate too, here are the rest of the Fall 2008 Semester GTGs:
Gizmo 1
Gizmo 2
Gizmo 3

Sunday, September 21, 2008

Swabbing the Decks and Guess That Gizmo

So I've been thinking about doing this for awhile now, debating it with myself as to if it would be an interesting and educational interactive series or if it would just cause more of what I call microphobia, and then there is the possibility no one will be interested. Microphobia is the term I use to describe the irrational fear of bacteria/microbes/germs. You see the vast majority of bacteria and other microorganisms are harmless and a lot are in fact beneficial and we need them. Within the human body there are more bacterial cells than there are human cells, I think that is so cool (yes I am a total nerd, this is well established). Microbes are used to make food and beverages, like bread, beer, wine, yogurt, some sausages, cheese, etc.

Diabetics can thank bacteria for their insulin, back in the day they used to extract insulin from animals like goats, horses and sheep, this would work for awhile but eventually the human immune system would mount an attack on the foreign substance. Knowing the structure of human insulin scientists converted the protein structure into DNA code and inserted it into E. coli* which turned it into an insulin factory and diabetics no longer have to worry about running out of animals their body hasn't already rejected. Of course there are bad microbes out there that can cause disease, spoil food (which can be good**) and other undesirable things, but they are in the minority. Even the bad can be good, Clostridium botulinum, the causative agent of botulism, makes the most potent toxin known to man but it has medicinal and cosmetic uses***.

What's the point? Oh yeah, I had a point! The point is that sometimes I have a plate or 10 that doesn't grow, normally I would just throw it away since it is not considered good technique to try and plate a new sample on it. I also have Q-tips that I could toss in the sterilizer. And so dear readers if you are curious as to what is present on some surface that I have access to and isn't obscene I am willing to swab it, if and when I have the time, and if anything cool grows I'll take a picture and write a post about it and give you some linky love if you have a blog. So if you think this is a cool idea and you have something in mind leave a comment to this post telling me what you want swabbed, if it is something I think is disturbing or gross (like naughty bits, besides I can already tell you boy parts usually are covered in Staphylococcus and girl parts have mainly lactic acid bacteria) it won't be published. Bacteria and fungi are pretty much everywhere so it is highly likely something will grow, I do however use a media that is made for lactic acid bacteria and not all microbes are culturable (most aren't) so I won't guarantee something will grow but most likely something will. Oh and I do not have the time nor the supplies to identify the organism(s) that grows, sorry.

And it has been awhile (again) so here is the next Guess that Gizmo:
Remember, Guess That Gizmo is a semester long contest, here are the other Fall 2008 GTG's:
Fall 2008 1
Fall 2008 2
Rules





-----Copy LiteralDan Section-----
*E. coli is usually a harmless organism that most people are colonized with, there are a few pathogenic strains which of course can cause disease but the vast majority of E. coli strains are harmless and potentially beneficial.
**While food spoilage is generally a bad thing it does have the benefit of preventing people from eating potentially dangerous food.
***Botox is botulinum toxin, it can be used to treat spastic disorders, excessive sweating, and of course the more famous use would be reducing wrinkles. Personally I think it is insane to inject that toxin into someone for cosmetic reasons, there have been deaths associated with it as I recall. 1mg of the toxin is theoretically capable of killing 1,000,000 hamsters/guinea pigs (I have heard of this study not seen it).

Friday, September 5, 2008

Guess that Gizmo 9-5-08

It is time for Guess that Gizmo, sorry that it has been so long between posts (both GTG posts and regular posts), I have been busy with the issues at work and also trying to catch up with my blog reading. To make it up to you I am adding a bonus to this one, same rules apply as the GTG rules, the bonus picture is Guess that Goo. For those just tuning in and who want to get in on the fun you still can totally catch up, the first GTG of the semester is here. I will probably tally the scores at the end of the semester. Remember this is totally for fun, the prize(s) will be blog bling (I have until December how to figure out how to make something into blog bling...) Clicking the images will show them larger if that helps.

Gizmo:
Goo:For the goo, guess what the white stuff floating in the tube is.

Have fun and good luck =)!

Saturday, August 16, 2008

Guess that Gizmo


Way back when I introduced (somewhat prematurely) what I plan to have be a regular feature on this blog: Guess that Gizmo, I hadn't even convinced Baby Sibling to make an awesome graphic for me (check out that awesomeness, thanks Baby Sibling!!!!!). I've been contemplating how I wanted this to work and decided that I am going to borrow some concepts from The Roaming Southerner and have it be a contest where people earn points over a given time frame and at the end someone gets a nerdy piece of blog bling that they can display proudly if they so choose (the time frame will give me time to figure out how blog bling works...). So here are the official Guess that Gizmo rules:

1. Guess that Gizmo will follow an academic calendar give or take:
  • Fall Semester: Late August through Mid December.
  • Spring Semester: Mid January through Early May.
  • Summer Semester*: Mid May through Mid August.
2. The point system is as follows:
  • Wrong but amusing answer: 1 point
  • Right answer: 2 points
  • I will not be giving the right answer until the end of the semester so more than one person can be right, for multiples of the wrong answer(s) only the first person gets the creativity point(s).
3. There is currently no limit on the number of guesses, if a miracle happens and I get swamped I may need to revisit this one.

4. Guess that Gizmo is meant to be fun, don't take it too seriously! There are things I encounter in labs that I have no idea what they are and sometimes try to guess what it is/does, figured that it might be fun to see what other people might guess.

5. And since one never knows: I am not making any statement for or against a brand/product/company by including it or its competitor in Guess that Gizmo (though I will totally sell out for an anaerobic incubator**! I am NOT kidding!) and my employer has absolutely nothing to do with the content of this blog and it is written on my personal computer.


*As a grad student I do not get the summer off, school is year round for us, so why should you all get the summer off Guess that Gizmo? ;) (LiteralDan's use of footnotes amuses me greatly and so I wanna be cool like him and use them on my blog, citing my source makes it totally ethical to blatantly copy him)

**Anaerobic means without oxygen, the bacteria I work with are anaerobes and will not grow in the presence of oxygen so I have to put my cultures in airtight jars with expensive packets that convert the oxygen into carbon dioxide and/or water, pain in the butt and limiting. An anaerobic incubator is pretty much #1 on my wish list. I have come close to having that dream come true, once it was the wrong thing and broken anyway (that was soooo embarrassing, though it tricked Dr. M too) and the next Boss Lady decided we needed a food grade centrifuge (machine that spins samples so that the solids in a liquid go to the bottom and stay there as the liquid is poured off) more than we needed the most awesomest incubator in the world (she is right, part of the project calls for people to sample what I make and so I need equipment that has never been in the path lab to get the study approved by the ethics board, she is not cool with my idea that we could just recruit people we don't like for the taste panel...).

Monday, August 4, 2008

Sunday Stylin'


What does a stylin' microbiologist wear to work on a Sunday you ask? Why she wears her matching GIANTmicrobes shirt and hat of course. I am not much of a hat wearer but I wanted to get a picture of me in the shirt and hat for my mommy since she bought them for me. I am totally in love with the giant microbes, even though they are totally off in saying they are a million times actual size with the majority of them. I am pretty sure I have them all and most of the new miniature ones. I can be a pain in the butt to buy presents for since I started working, I usually buy whatever I want and so there is nothing in particular to tell family members what to get me. I bought all the original giant microbes but since then I don't buy them (Mom would kick my butt if I did!), they are strictly reserved for the family.

Anyone want to make a guess as to what I am posing next to? Leave your guess(es) in the comments, there are points for creativity and amusement factor, and of course points if you get it right =)!