The two dilutions I commonly make are 1 in 100 (1 part of sample into 99 parts diluent) and 1 in 10. When I plate the sample I plate 0.1 ml so that adds another 1 in 10 dilution in the end. I multiply the number of colonies on the resultant plates by the number of times I diluted and it gives me the number of cells per ml in the original sample. Not diluted enough the bacteria form a smear across the plate, if too diluted then only a few colonies if any are present, I want approximately 20-250 colonies to have what is considered statistically significant results. And at risk of losing all of my beloved readers and having Baby Sibling make fun of how incredibly dorky I am I have made a video of me doing a dilution set and spread plating it:
Sorry about the not so sticky biohazard sticker, they just don't make 'em like they used to I guess:
And remember, this is a semester long contest so new readers can participate too, here are the rest of the Fall 2008 Semester GTGs:
Gizmo 1
Gizmo 2
Gizmo 3
9 comments:
i'm gonna take a wild guess and say an incubator. seems like an appropriate guess since that was the next step in ur slide vid and you didnt show it to us :-P
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