Dear Research Organisms,
You know that I love you all and enjoy working with you for the most part, yeah sometimes I get jealous of the others with their bugs* that do not have to be incubated without oxygen like you but all in all you are great bugs. The fact that first B. breve, my shining star bug, stopped growing after the initial revival step. That was bad enough but then when I got up early so I could inoculate for the last run of my last rep (not counting redoing some runs that did not turn out after I am done with rep 3), I was so excited! Then I pulled you out of the incubator and only B. bifidum grew well, B. breve grew a tiny bit, and B. infantis and B. longum, nothing!
It makes absolutely no sense that you did not grow, you grew in the first step and that was coming out of the -75°C freezer. It would make some sense if you did not revive at that point but the second pass, why!?! I am trying again but this puts me way off my schedule! Thankfully the new schedule works so that I will still be able to see Doc on Monday, though of course I am quite nervous about this appointment since it is basically a one way ticket to referral land and I do NOT want to go there! You all are so not helping, you know stress is a trigger for GI issues right? I will admit, I stress you out with my simulated GI tract, I'm sorry but it is for your own good. Have you all gone bad and made some sort of deal with Helicobacter pylori??? Giving me an ulcer will make this take longer but it is not going to stop me so you might as well be good little bugs and grow like you are supposed to!
~~~~~Copy LiteralDan Section~~~~~
*Bugs means bacteria or other microorganisms, it is a somewhat common nickname in my field and I use it a lot.
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